Project
CMV + GFP

Part:BBa_K1852000:Design

Designed by: Manuel Lerma   Group: iGEM15_Tec-Chihuahua   (2015-09-18)


GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1230


Design Notes

The composite part was designed to be placed within the plasmid psB1T3 since the basic parts that make it up contain resistance to chloranphenicol and ampicilin. This was done to be able to use the 3A assembly protocol.